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KMID : 0613820190290101152
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Journal of Life Science
2019 Volume.29 No. 10 p.1152 ~ p.1158
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Inhibitory Effect of the Ethanol Extract of Rudbeckia laciniata var. hortensis Bailey on Adipocyte Differentiation in 3T3-L1 Cells
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Nam Gun-He
Wee Ji-Hyang
Kim Sang-Yong
Baek Ji-Young
Kim Young-Min
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Abstract
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Rudbeckia laciniata var. hortensis Bailey is used in home remedy for colic and gastritis in South Korea. Although Rudbeckia laciniata var. hortensis Bailey is used extensively for home remedies, no single study on its efficacy exists. In this study, we investigated the anti-obesity effects of Rudbeckia laciniata var. hortensis Bailey. The anti-obesity effect of a 70% ethanol extract from Rudbeckia laciniata var. hortensis Bailey on the differentiation of 3T3-L1 pre-adipocytes to adipocytes was investigated with an Oil Red O assay, western blot analysis, and mRNA analysis. Compared to the control (only treated with DM), the 70% ethanol extract of Rudbeckia laciniata var. hortensis Bailey significantly inhibited adipocyte differentiation and intracellular triglyceride (TG) levels at a concentration of 100 μg/ml. To determine how the TG content was reduced, we measured the level of protein and mRNA expression of obesity- related agents, such as peroxisome proliferators?activated receptor γ (PPARγ), CCAAT/enhancer- binding protein α (C/EBPα), AMP-activated protein kinase (AMPK) phosphorylation, LPL, and FAS. As a result, the 70% ethanol extract of Rudbeckia laciniata var. hortensis Bailey significantly increased the expression of AMPK and decreased the expression of genes related to adipogenesis and fat storage, such as PPARγ, C/EBPα, LPL, and FAS.
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KEYWORD
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Adipocytes, C/EBPα, FAS, PPARγ, Rudbeckia laciniata var. hortensis Bailey
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